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Molecular characterization and expression analysis of Cyclin B and Cell division cycle 2 in gonads of diploid and triploid bighead catfish, Clarias macrocephalus Günther, 1864
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Metadata
Document Title
Molecular characterization and expression analysis of Cyclin B and Cell division cycle 2 in gonads of diploid and triploid bighead catfish, Clarias macrocephalus Günther, 1864
Author
Wachirachaikarn A., Rungsin W., Srisapoome P., Klinbunga S., Na-Nakorn U.
Name from Authors Collection
Affiliations
Interdisciplinary Program in Genetic Engineering, Graduate School, Kasetsart University, Bangkok, 10900, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, 10900, Thailand; Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Agriculture and Natural Resources
ISSN
24681458
Year
2017
Volume
51
Issue
2
Page
117-130
Open Access
Hybrid Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.anres.2016.05.004
Abstract
This study investigated the differential expression of genes associated with reproduction in sterile triploid and normal diploid bighead catfish (Clarias macrocephalus Günther, 1864). The triploid fish were produced using cold shock and were reared in the same conditions as the diploid counterpart. The histomicrographs showed completely retarded triploid gonads across the samples aged 2–12 mth, whereas the gonads of the diploids were in developing stages during 2–4 mth, reached the early maturing stage at 6 mth, matured at 8 mth and showed signs of atresia at 10–12 mth. In parallel, the full-length cDNAs of cyclin B1 (CmCcnb1; 1539 bp in length with an open reading frame (ORF) of 1194 bp corresponding to 397 amino acids) and cell division cycle 2 (CmCdc2; 1355 bp, an ORF of 909 bp, 302 amino acids) of bighead catfish (C. macrocephalus Günther, 1864) were isolated. Phylogenetic analysis revealed that the newly characterized CmCcnb1 should be regarded as a member of cyclin B1 rather than cyclin B2. The expression level of CmCcnb1 mRNA was limited in different stages of the ovaries and testes of triploids. In diploid ovaries, its expression was significantly higher than that in triploid ovaries in fish aged 2 mth (513.43 ± 82.22 fold) and in fish aged 8 mth (2430.87 ± 900.06 fold). The CmCcnb1 level in the testes of diploids was significantly greater than that in triploids in fish aged 2 mth (928.85 ± 208.72 fold). Similarly, expression of CmCdc2 mRNA was also reduced in triploids. Its expression was significantly lower than that in diploid females aged 2 mth (7.66 ± 3.42 fold), 4 mth (59.42 ± 10.50 fold) and 8 mth (42.74 ± 8.36 fold). In males, significantly greater expression of CmCdc2 was observed at age 6 mth (58.61 ± 34.64 fold) and 8 mth (72.70 ± 4.36 fold) diploids compared to triploids. The results illustrated that CmCcnb1 and CmCdc2 are functionally involved in oogenesis and spermatogenesis and reduced expression levels of these transcripts affected the reproductive development of triploid C. macrocephalus. © 2017 Kasetsart University
Keyword
Cdc2 | Clarias macrocephalus | Cyclin B | Diploids | Expression | Triploids
Funding Sponsor
Office of the Higher Education Commission; National Science and Technology Development Agency; Kasetsart University Research and Development Institute; National Center for Genetic Engineering and Biotechnology
License
CC BY-NC-ND
Rights
Kasetsart University
Publication Source
Scopus