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Molecular epidemiology of Sporothrix schenkii isolates in Malaysia
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Document Title
Molecular epidemiology of Sporothrix schenkii isolates in Malaysia
Author
Azam NKK, Selvarajah GT, Santhanam J, Razak MFA, Ginsapu SJ, James JE, Suetrong S
Name from Authors Collection
Scopus Author ID
16319823700
Affiliations
Universiti Kebangsaan Malaysia; Universiti Putra Malaysia; Kementerian Kesihatan Malaysia; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
MEDICAL MYCOLOGY
ISSN
1369-3786
Year
2020
Volume
58
Issue
5
Page
617-625
Open Access
Green Accepted
Publisher
OXFORD UNIV PRESS
DOI
10.1093/mmy/myz106
Format
Abstract
Sporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Funding Sponsor
Faculty of Veterinary Medicine, Universiti Putra Malaysia
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Publication Source
WOS