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Molecular Insights into the Mode of Action of Antibacterial Peptides Derived from Chicken Plasma Hydrolysates
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Document Title
Molecular Insights into the Mode of Action of Antibacterial Peptides Derived from Chicken Plasma Hydrolysates
Author
Tian F, Rodtong S, Thumanu K, Hua YL, Roytrakul S, Yongsawatdigul J
Name from Authors Collection
Affiliations
Suranaree University of Technology; Suranaree University of Technology; Suranaree University of Technology; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
FOODS
Year
2022
Volume
11
Issue
22
Page
-
Open Access
Green Published, gold
Publisher
MDPI
DOI
10.3390/foods11223564
Format
Abstract
Due to the overuse and abuse of antibiotics, several antibiotic resistant bacteria have emerged. Antimicrobial peptides (AMPs) have gained attention as alternative antimicrobial agents because of their unique mode of action that impedes bacterial resistance. Two novel antibacterial peptides were isolated from Alcalase-hydrolyzed chicken plasma by size exclusion and reverse-phase chromatography. They were identified by LC-MS/MS to be VSDH and CCCPKAF, which showed effective antibacterial activity toward Bacillus cereus DMST 5040, with varied modes of action. The peptide CCCPKAF caused cell membrane disintegration, as evidenced by propidium iodide (PI) uptake. In contrast, the peptide VSDH targeted intracellular molecules, including proteins and nucleic acids, as revealed by Synchrotron-based Fourier Transform Infrared (SR-FTIR). The secondary structure of intracellular proteins increased to a beta-sheet structure concomitant with a decrease in the alpha-helix structure when exposed to 0.5 mM VSDH. Molecular docking analysis revealed that VSDH showed high binding affinity for the active sites of the various enzymes involved in DNA synthesis. In addition, it showed good affinity for a chaperone protein (Dnak), resulting in the misfolding of intracellular proteins. Nuclear magnetic resonance (NMR) and molecular dynamics simulations also indicated that VSDH chelated well with Mg2+, which could partly contribute to its antibacterial activity.
Keyword
antibacterial peptides | Bacillus cereus | molecular docking | SR-FTIR
Funding Sponsor
National Science and Research Innovation Fund (NSRF) under the Fundamental Fund Project [FF3-305-65-12-33(08)]; National Research Council of Thailand [N42A650548]; National Council of Thailand; Suranaree University of Technology
License
CC-BY
Rights
Authors
Publication Source
WOS