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Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice
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Metadata
Document Title
Molecular Markers and Candidate Genes for Thermo-Sensitive Genic Male Sterile in Rice
Author
Khlaimongkhon S., Chakhonkaen S., Pitngam K., Ditthab K., Sangarwut N., Panyawut N., Wasinanon T., Mongkolsiriwatana C., Chunwongse J., Muangprom A.
Name from Authors Collection
Scopus Author ID
24467446600
Scopus Author ID
56353145600
Scopus Author ID
57194215247
Scopus Author ID
57194206928
Affiliations
Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom, 73140, Thailand; National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Division of Genetics, Faculty of Liberal Arts and Science, Kasetsart University, Nakhon Pathom, 73140, Thailand
Type
Article
Source Title
Rice Science
ISSN
16726308
Year
2019
Volume
26
Issue
3
Page
147-156
Open Access
Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.rsci.2018.08.006
Format
Abstract
The discovery of thermo-sensitive genic male sterility (TGMS)has led to development of a simple and highly efficient two-line breeding system. In this study, genetic analysis was conducted using three F2 populations derived from crosses between IR68301S, an indica TGMS rice line, and IR14632 (tropical japonica), Supanburi 91062 (indica)and IR67966-188-2-2-1 (tropical japonica), respectively. Approximately 1:3 ratio between sterile and normal pollen of F2 plants from the three populations revealed that TGMS is controlled by a single recessive gene. Bulked segregant analysis using simple sequence repeat (SSR)and insertion-deletion (InDel)markers were used to identify markers linked to the tms gene. The linkage analysis based on the three populations indicated that the tms locus was located on chromosome 2 covering the same area. Using IR68301S × IR14632 F2 population, the results showed that the tms locus was located between SSR marker RM12676 and InDel marker 2gAP0050058. The genetic distance from the tms gene to these two flanking markers were 1.10 and 0.82 cM, respectively. InDel marker 2gAP004045 located between these two markers showed complete co-segregation with the TGMS phenotype. In addition, InDel marker vf0206114052 showed 2.94 cM linked to the tms gene using F2 populations of IR68301S × Supanburi 91062. These markers are useful tool for developing new TGMS lines by marker-assisted selection. There were ten genes located between the two flanking markers RM12676 and 2gAP0050058. Using quantitative real-time PCR for expression analysis, 7 of the 10 genes showed expression in panicles, and response to temperatures. These genes could be the candidate gene controlling TGMS in IR68301S. © 2019 China National Rice Research Institute
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; Agricultural Research Development Agency
License
CC BY-NC
Rights
Author
Publication Source
Scopus
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