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Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection
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Document Title
Multi-color fluorescent reporter dengue viruses with improved stability for analysis of a multi-virus infection
Author
Suphatrakul A, Duangchinda T, Jupatanakul N, Prasittisa K, Onnome S, Pengon J, Siridechadilok B
Name from Authors Collection
Affiliations
National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC)
Type
Article
Source Title
PLOS ONE
ISSN
1932-6203
Year
2018
Volume
13
Issue
4
Open Access
Green Published, gold, Green Submitted
Publisher
PUBLIC LIBRARY SCIENCE
DOI
10.1371/journal.pone.0194399
Format
Abstract
Reporter virus is a versatile tool to visualize and to analyze virus infections. However, for flaviviruses, it is difficult to maintain the inserted reporter genes on the viral genome, limiting its use in several studies that require homogeneous virus particles and several rounds of virus replication. Here, we showed that flanking inserted GFP genes on both sides with ribosome-skipping 2A sequences improved the stability and the consistency of their fluorescent signals for dengue-virus-serotype 2 (DENV2) reporter viruses. The reporter viruses can infect known susceptible mammalian cell lines and primary CD14+ human monocytes. This design can accommodate several fluorescent protein genes, enabling the generation of multi-color DENV2-16681 reporter viruses with comparable replication capabilities, as demonstrated by their abilities to maintain their fluorescent intensities during co-infections and to exclude superinfections regardless of the fluorescent tags. The reported design of multi-color DENV2 should be useful for high-throughput analyses, single-cell analysis, and characterizations of interference and superinfection in animal models.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Grand Challenges Canada, Stars in Global Health Program Round 5
License
CC BY
Rights
Authors
Publication Source
WOS