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Nucleocapsid proteins from other swine enteric coronaviruses differentially modulate PEDV replication
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Document Title
Nucleocapsid proteins from other swine enteric coronaviruses differentially modulate PEDV replication
Author
Sungsuwan S., Jongkaewwattana A., Jaru-Ampornpan P.
Name from Authors Collection
Affiliations
Virology and Cell Technology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)Pathum Thani 12120, Thailand
Type
Article
Source Title
Virology
ISSN
00426822
Year
2020
Volume
540
Page
45-56
Open Access
All Open Access, Bronze, Green
Publisher
Academic Press Inc.
DOI
10.1016/j.virol.2019.11.007
Format
Abstract
Porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV) and porcine deltacoronavirus (PDCoV) share tropism for swine intestinal epithelial cells. Whether mixing of viral components during co-infection alters pathogenic outcomes or viral replication is not known. In this study, we investigated how different coronavirus nucleocapsid (CoV N) proteins interact and affect PEDV replication. We found that PDCoV N and TGEV N can competitively interact with PEDV N. However, the presence of PDCoV or TGEV N led to very different outcomes on PEDV replication. While PDCoV N significantly suppresses PEDV replication, overexpression of TGEV N, like that of PEDV N, increases production of PEDV RNA and virions. Despite partial interchangeability in nucleocapsid oligomerization and viral RNA synthesis, endogenous PEDV N cannot be replaced in the production of infectious PEDV particles. Results from this study give insights into functional compatibilities and evolutionary relationship between CoV viral proteins during viral co-infection and co-evolution. © 2019
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Number
P-15-51261
License
N/A
Rights
N/A
Publication Source
Scopus