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Oroxylin A shows limited antiviral activity towards dengue virus
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Document Title
Oroxylin A shows limited antiviral activity towards dengue virus
Author
Ratanakomol T., Roytrakul S., Wikan N., Smith D.R.
Name from Authors Collection
Affiliations
Institute of Molecular Biosciences, Mahidol University, Salaya, 73170, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
Type
Article
Source Title
BMC Research Notes
ISSN
17560500
Year
2022
Volume
15
Issue
1
Open Access
Gold, Green
Publisher
BioMed Central Ltd
DOI
10.1186/s13104-022-06040-0
Format
Abstract
Objective: The mosquito transmitted dengue virus (DENV) the causative agent of dengue fever (DF) remains a significant public health burden in many countries. Thailand, along with many countries in Asia and elsewhere, has a long history of using traditional medicines to combat febrile diseases such as DF. Screening bioactive compounds from traditional medicines reported to have antipyretic or anti-inflammatory activity may lead to the development of potent antivirals. In this study oroxylin A (OA), a flavonoid derivative found in Oroxylum indicum (commonly called the Indian trumpet flower or tree of Damocles), was screened for antiviral activity towards DENV. Results: Cytotoxicity analysis in BHK-21 cells showed a 50% cytotoxic concentration (CC50) of 534.17 µM. The compound showed no direct virucidal activity towards DENV, and pre-treatment of cells had no effect on virus production. A deficit was seen in virus production when cells were post-infection treated with oroxylin A. Under conditions of post-infection treatment, the EC50 value was 201.1 µM, giving a selectivity index (SI) value of 2.66. Accumulation of DENV E protein inside the cell was seen under conditions of post-infection treatment, suggesting that oroxylin A may exert some effects at the virus assembly/egress stages of the replication cycle. © 2022, The Author(s).
Keyword
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Funding Sponsor
Centre of Excellence in Mathematics, Mahidol University; Mahidol University: NDFR 28/2564; National Science and Technology Development Agency; National Research Council of Thailand: NRCT5-RSA63015-03; Faculty of Tropical Medicine, Mahidol University; Faculty of Science, Mahidol University; Thailand Graduate Institute of Science and Technology: SCA-CO-2561-6979TH; Faculty of Medicine Siriraj Hospital, Mahidol University; Department of Physiology, Mahidol University; Faculty of Information and Communication Technology, Mahidol University
License
CC BY
Rights
Author
Publication Source
Scopus