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OsBTBZ1 Confers Salt Stress Tolerance in Arabidopsis thaliana
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Document Title
OsBTBZ1 Confers Salt Stress Tolerance in Arabidopsis thaliana
Author
Saputro T.B. Jakada B.H. Chutimanukul P. Comai L. Buaboocha T. Chadchawan S.
Affiliations
Center of Excellence in Environment and Plant Physiology Department of Botany Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Program in Biotechnology Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; National Center for Genetic Engineering and Biotechnology National Science and Technology Development Agency Khlong Luang Pathumthani Bangkok 12120 Thailand; Genome Center Department of Plant Biology UC Davis Davis CA 95616 United States; Center of Excellence in Molecular Crop Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Omics Science and Bioinformatics Center Faculty of Science Chulalongkorn University Bangkok 10330 Thailand
Type
Article
Source Title
International Journal of Molecular Sciences
ISSN
16616596
Year
2023
Volume
24
Issue
19
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/ijms241914483
Abstract
Rice (Oryza sativa L.) one of the most important commodities and a primary food source worldwide can be affected by adverse environmental factors. The chromosome segment substitution line 16 (CSSL16) of rice is considered salt-tolerant. A comparison of the transcriptomic data of the CSSL16 line under normal and salt stress conditions revealed 511 differentially expressed sequence (DEseq) genes at the seedling stage 520 DEseq genes in the secondary leaves and 584 DEseq genes in the flag leaves at the booting stage. Four BTB genes OsBTBZ1 OsBTBZ2 OsBTBN3 and OsBTBN7 were differentially expressed under salt stress. Interestingly only OsBTBZ1 was differentially expressed at the seedling stage whereas the other genes were differentially expressed at the booting stage. Based on the STRING database OsBTBZ1 was more closely associated with other abiotic stress-related proteins than other BTB genes. The highest expression of OsBTBZ1 was observed in the sheaths of young leaves. The OsBTBZ1-GFP fusion protein was localized to the nucleus supporting the hypothesis of a transcriptionally regulatory role for this protein. The bt3燗rabidopsis mutant line exhibited susceptibility to NaCl and abscisic acid (ABA) but not to mannitol. NaCl and ABA decreased the germination rate and growth of the mutant lines. Moreover the ectopic expression of OsBTBZ1 rescued the phenotypes of the bt3 mutant line and enhanced the growth of wild-type Arabidopsis under stress conditions. These results suggest that OsBTBZ1 is a salt-tolerant gene functioning in ABA-dependent pathways. ? 2023 by the authors.
Keyword
abiotic stress | abscisic acid | BTB domain | BTBZ | Salt stress | tolerance
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus