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Document Title

Pharmacokinetics behavior of four cannabidiol preparations following single oral administration in dogs

Author

Limsuwan S., Phonsatta N., Panya A., Asasutjarit R., Tansakul N.

Affiliations

Interdisciplinary Graduate Programs in Bioscience, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Colin Rateledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zibo, 255000, China; Omics Center for Agriculture, Bioresources, Food, and Health Kasetsart University (OmiKU), Bangkok, 10900, Thailand; Institute of Nutrition, Mahidol University, Nakhon Pathom, 73170, Thailand; Department of Food Sciences, College of Food Science and Engineering, Lingnan Normal University, Zhanjiang, 524048, China; College of Ecology, Resources and Environment, Dezhou University, Dezhou, 253000, China; Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China; Industrial Bioprocess Technology Research Team, Functional Ingredient and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

Type

Article

Source Title

Biology

ISSN

20797737

Year

2024

Volume

13

Issue

4

Open Access

All Open Access, Gold

Publisher

Multidisciplinary Digital Publishing Institute (MDPI)

DOI

10.3390/biology13040276

Abstract

Mucor circinelloides WJ11 is a lipid-producing strain with industrial potential. A holistic approach using gene manipulation and bioprocessing development has improved lipid production and the strain’s economic viability. However, the systematic regulation of lipid accumulation and carotenoid biosynthesis in M. circinelloides remains unknown. To dissect the metabolic mechanism underlying lipid and carotenoid biosynthesis, transcriptome analysis and reporter metabolites identification were implemented between the wild-type (WJ11) and ?carRP WJ11 strains of M. circinelloides. As a result, transcriptome analysis revealed 10,287 expressed genes, with 657 differentially expressed genes (DEGs) primarily involved in amino acid, carbohydrate, and energy metabolism. Integration with a genome-scale metabolic model (GSMM) identified reporter metabolites in the ?carRP WJ11 strain, highlighting metabolic pathways crucial for amino acid, energy, and nitrogen metabolism. Notably, the downregulation of genes associated with carotenoid biosynthesis and acetyl-CoA generation suggests a coordinated relationship between the carotenoid and fatty acid biosynthesis pathways. Despite disruptions in the carotenoid pathway, lipid production remains stagnant due to reduced acetyl-CoA availability, emphasizing the intricate metabolic interplay. These findings provide insights into the coordinated relationship between carotenoid and fatty acid biosynthesis in M. circinelloides that are valuable in applied research to design optimized strains for producing desired bioproducts through emerging technology. ? 2024 by the authors.

License

CC BY

Rights

Authors

Publication Source

WoS

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