-
Phylogenetic and chemotaxonomic studies confirm the affinities of stromatoneurospora phoenix to the coprophilous xylariaceae
- Back
Document Title
Phylogenetic and chemotaxonomic studies confirm the affinities of stromatoneurospora phoenix to the coprophilous xylariaceae
Author
Becker K., Wongkanoun S., Wessel A.-C., Bills G.F., Stadler M., Luangsa-Ard J.J.
Name from Authors Collection
Scopus Author ID
57189995748
Affiliations
Department of Microbial Drugs, Helmholtz Centre for Infection Research GmbH (HZI), Inhoffenstraße 7, Braunschweig, 38124, Germany; German Centre for Infection Research Association (DZIF), Partner Site Hannover-Braunschweig, Inhoffenstraße 7, Braunschweig, 38124, Germany; Faculty of Biotechnology, College of Agricultural Innovation, Biotechnology and Food, Rangsit University, Phahonyothin Road, Lak-Hok, Pathum Thani, 12000, Thailand; National Biobank of Thailand (NBT), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand; Texas Therapeutics Institute, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center at Houston, 1881 East Road, Houston, TX 77054, United States; Plant Microbe Interaction Research Team (APMT), Integrative Crop Biotechnology and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Phahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani 12120, Thailand
Type
Article
Source Title
Journal of Fungi
ISSN
2309608X
Year
2020
Volume
6
Issue
3
Page
Jan-21
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/jof6030144
Abstract
The genus Stromatoneurospora was erected in 1973 by Jong and Davis to accommodate the pyrophilic pyrenomycete Sphaeria phoenix and has traditionally been placed in the family Xylariaceae based on morphological features. However, no living culture of this genus has so far been available in the public domain. Molecular data were restricted to an internal transcribed spacer (ITS) sequence that only confirmed the familial position, and was generated from a strain that is not deposited in a public culture collection. We have recently collected fresh material and were able to culture this fungus from Thailand. The secondary metabolites of this strains were analysed after fermentation in multiple media. The the prominent components of these fermentation were purified, using preparative chromatography. Aside from two new eremophilane sesquiterpenoids named phoenixilanes A–B (1–2), four other components that are known from species of the xylariaceous genera Xylaria and Poronia were identified by spectral methods (nuclear magnetic resonance spectroscopy and high resolution mass spectrometry). Notably, (−)-(R)-6-hydroxy-3-methyl-4-dihydroisocoumarin-5-carboxylic acid (6) has not been reported as a natural product before. Moreover, DNA sequences of Stromatoneurospora phoenix clustered with members of the genera Poronia and Podosordaria in a multi-locus molecular phylogeny. These results confirmed that the genus belongs to the same evolutionary lineage as the coprophilic Xylariaceae. The results also suggest that this lineage has evolved independently from the plant-inhabiting saprotrophs and endophytes that are closely related to the genus Xylaria. These findings are discussed in relation to some theories about the endophytic vs. the pyrophilic/coprophilic fungal life style. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
National Institutes of Health; Horizon 2020 Framework Programme; Deutsche Forschungsgemeinschaft; Horizon 2020
License
CC BY
Rights
Author
Publication Source
Scopus