Abstract
A novel actinomycete strain, A-T 11038(T), was isolated from bamboo rhizospheric soil collected in Thailand. Based on a polyphasic approach, the novel strain was characterized as a member of the genus Planomonospora, which developed cylindrical to clavate sporangia containing a single motile spore on aerial mycelium. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 11038(T) was closely related to Planomonospora sphaerica JCM 9374(T) (98.82 %), P. lanomonospora parontospora subsp. parontospora NBRC 13880(T) and P. parontospora subsp. antibiotica JCM 3094(T) (98.54 %), Planomonospora alba JCM 9373(T) (98.41 %) and Planomonospora venezuelensis JCM 3167(T) (97.51 %). The DNA-DNA relatedness values that distinguished strain A-T 11038(T) from the most closely related species were below 45 %. The novel strain contained meso-diaminopimelic acid in cell-wall hydrolysates, and rhamnose, ribose, madurose and glucose in whole-cell hydrolysates. The predominant menaquinone was MK-9(H-2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol and aminophosphoglycolipids. The predominant cellular fatty acids were unsaturated fatty acids C-17 : 1 and C-16 : 1 and saturated fatty acid C-16 : 0. The G+C content of the genomic DNA was 73.5 mol%. Following the evidence obtained using a polyphasic approach, the novel strain is proposed as a representative of a novel species to be named Planomonospora corallina sp. nov. The type strain is A-T 11038(T) (=BCC 67829(T) =TBRC 4489(T) =NBRC 110609(T)).
