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Plant-Produced Receptor-Binding Domain of SARS-CoV-2 Elicits Potent Neutralizing Responses in Mice and Non-human Primates
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Metadata
Document Title
Plant-Produced Receptor-Binding Domain of SARS-CoV-2 Elicits Potent Neutralizing Responses in Mice and Non-human Primates
Author
Siriwattananon K.,Manopwisedjaroen S.,Shanmugaraj B.,Rattanapisit K.,Phumiamorn S.,Sapsutthipas S.,Trisiriwanich S.,Prompetchara E.,Ketloy C.,Buranapraditkun S.,Wijagkanalan W.,Tharakhet K.,Kaewpang P.,Leetanasaksakul K.,Kemthong T.,Suttisan N.,Malaivijitnond S.,Ruxrungtham K.,Thitithanyanont A.,Phoolcharoen W.
Name from Authors Collection
Affiliations
Research Unit for Plant-produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand; BaiyaPhytopharm Co., Ltd, Bangkok, Thailand; Department of Medical Sciences, Ministry of Public Health, Institute of Biological Products, Nonthaburi, Thailand; Faculty of Medicine, Center of Excellence in Vaccine Research and Development (Chula Vaccine Research Center, Chula VRC), Chulalongkorn University, Bangkok, Thailand; Department of Laboratory Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Department of Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; BioNet-Asia Co., Ltd, Bangkok, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, Pathum Thani, Thailand; National Primate Research Center of Thailand-Chulalongkorn University, Saraburi, Thailand
Type
Article
Source Title
Frontiers in Plant Science
ISSN
1664462X
Year
2021
Volume
12
Open Access
All Open Access, Gold, Green
Publisher
Frontiers Media S.A.
DOI
10.3389/fpls.2021.682953
Abstract
The emergence of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected global public health and economy. Despite the substantial efforts, only few vaccines are currently approved and some are in the different stages of clinical trials. As the disease rapidly spreads, an affordable and effective vaccine is urgently needed. In this study, we investigated the immunogenicity of plant-produced receptor-binding domain (RBD) of SARS-CoV-2 in order to use as a subunit vaccine. In this regard, RBD of SARS-CoV-2 was fused with Fc fragment of human IgG1 and transiently expressed in Nicotiana benthamiana by agroinfiltration. The plant-produced RBD-Fc fusion protein was purified from the crude extract by using protein A affinity column chromatography. Two intramuscular administration of plant-produced RBD-Fc protein formulated with alum as an adjuvant have elicited high neutralization titers in immunized mice and cynomolgus monkeys. Further it has induced a mixed Th1/Th2 immune responses and vaccine-specific T-lymphocyte responses which was confirmed by interferon-gamma (IFN-γ) enzyme-linked immunospot assay. Altogether, our results demonstrated that the plant-produced SARS-CoV-2 RBD has the potential to be used as an effective vaccine candidate against SARS-CoV-2. To our knowledge, this is the first report demonstrating the immunogenicity of plant-produced SARS-CoV-2 RBD protein in mice and non-human primates. © Copyright © 2021 Siriwattananon, Manopwisedjaroen, Shanmugaraj, Rattanapisit, Phumiamorn, Sapsutthipas, Trisiriwanich, Prompetchara, Ketloy, Buranapraditkun, Wijagkanalan, Tharakhet, Kaewpang, Leetanasaksakul, Kemthong, Suttisan, Malaivijitnond, Ruxrungtham, Thitithanyanont and Phoolcharoen.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Author
Publication Source
Scopus