-
PmTBC1D20, a Rab GTPase-activating protein from the black tiger shrimp, Penaeus monodon, is involved in white spot syndrome virus infection
- Back
Document Title
PmTBC1D20, a Rab GTPase-activating protein from the black tiger shrimp, Penaeus monodon, is involved in white spot syndrome virus infection
Author
Yingvilasprasert W., Supungul P., Tassanakajon A.
Name from Authors Collection
Scopus Author ID
6508068998
Affiliations
Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani 12120, Thailand
Type
Article
Source Title
Developmental and Comparative Immunology
ISSN
0145305X
Year
2014
Volume
42
Issue
2
Page
302-310
Open Access
All Open Access, Bronze
DOI
10.1016/j.dci.2013.09.008
Format
Abstract
TBC domain family member 20 (TBC1D20) has recently been reported to mediate Hepatitis C virus replication. Herein, PmTBC1D20 identified from the black tiger shrimp, Penaeus monodon, was characterized and evaluated for its role in white spot syndrome virus (WSSV) infection. The full-length cDNA sequence of PmTBC1D20 contains 2003. bp with a predicted 1443. bp open reading frame encoding a deduced 480 amino acid protein. Its transcript levels were significantly up-regulated at 24 and 48. h by ~2.3- and 2.1-fold, respectively, after systemic infection with WSSV. In addition, depletion of PmTBC1D20 transcript in shrimps by double stranded RNA interference led to a decrease in the level of transcripts of three WSSV genes (VP28, ie1 and wsv477). This suggests the importance of PmTBC1D20 in WSSV infection. This is the first report of TBC1D20 in a crustacean and reveals the possible mechanism used by WSSV to modulate the activity of the host protein, PmTBC1D20, for its benefit in viral trafficking and replication. ? 2013 Elsevier Ltd.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Japan Science and Technology Agency; National Science and Technology Development Agency; Thailand Research Fund; Japan International Cooperation Agency; Thailand Graduate Institute of Science and Technology; Science and Technology Research Partnership for Sustainable Development
License
N/A
Rights
N/A
Publication Source
Scopus