-
Preliminary study: Proteomic profiling uncovers potential proteins for biomonitoring equine melanocytic neoplasm
- Back
Metadata
Document Title
Preliminary study: Proteomic profiling uncovers potential proteins for biomonitoring equine melanocytic neoplasm
Author
Tesena P., Kingkaw A., Vongsangnak W., Pitikarn S., Phaonakrop N., Roytrakul S., Kovitvadhi A.
Name from Authors Collection
Affiliations
Graduate Student in Animal Health and Biomedical Science Program, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand; Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Puttamonthon, Nakhon Pathom, 73170, Thailand; Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Department of Zoology, Faculty of Sciences, Kasetsart University, Bangkok, 10900, Thailand; Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), Bangkok, 10900, Thailand; Genetic Engineering and Bioinformatics Program, Graduate School, Kasetsart University, Bangkok, 10900, Thailand; Functional Ingredients and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand; Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand
Type
Article
Source Title
Animals
ISSN
20762615
Year
2021
Volume
11
Issue
7
Open Access
Gold, Green
Publisher
MDPI AG
DOI
10.3390/ani11071913
Abstract
Equine melanocytic neoplasm (EMN) is a cutaneous neoplasm and is mostly observed in aged grey horses. This preliminary study aimed to identify potential proteins to differentiate normal, mild and severe EMN from serum proteomic profiling. Serum samples were collected from 25 grey horses assigned to three groups: normal (free of EMN; n = 10), mild (n = 6) and severe EMN (n = 9). To explore the differences in proteins between groups, proteomic profiling and analysis were employed. Accordingly, 8241 annotated proteins out of 8725 total proteins were compared between normal and EMN groups and inspected based on differentially expressed proteins (DEPs). Through DEP analysis, 95 significant DEPs differed between normal and EMN groups. Among these DEPs, 41 significant proteins were categorised according to protein functions. Based on 41 significant proteins, 10 were involved in metabolism and 31 in non-metabolism. Interestingly, phospholipid phosphatase6 (PLPP6) and ATPase subunit alpha (Na+/K+-ATPase) were considered as potential proteins uniquely expressed in mild EMN and related to lipid and energy metabolism, respectively. Non-metabolism-related proteins (BRCA1, phosphorylase B kinase regulatory subunit: PHKA1, tyrosine-protein kinase receptor: ALK and rho-associated protein kinase: ROCK1) correlated to melanoma development differed among all groups. The results of our study provide a foundation for early EMN biomonitoring and prevention. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Funding Sponsor
National Science and Technology Development Agency
License
CC BY
Rights
Author
Publication Source
Scopus