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Production of high activity Aspergillus niger BCC4525 β-mannanase in Pichia pastoris and its application for mannooligosaccharides production from biomass hydrolysis
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Document Title
Production of high activity Aspergillus niger BCC4525 β-mannanase in Pichia pastoris and its application for mannooligosaccharides production from biomass hydrolysis
Author
Harnpicharnchai P., Pinngoen W., Teanngam W., Sornlake W., Sae-Tang K., Manitchotpisit P., Tanapongpipat S.
Name from Authors Collection
Scopus Author ID
6602764100
Affiliations
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, Thailand; Faculty of Science, Department of Medical Sciences, Rangsit University, Pathum Thani, Thailand
Type
Article
Source Title
Bioscience, Biotechnology and Biochemistry
ISSN
09168451
Year
2016
Volume
80
Issue
12
Page
2298-2305
Open Access
All Open Access, Bronze, Green
Publisher
Japan Society for Bioscience Biotechnology and Agrochemistry
DOI
10.1080/09168451.2016.1230003
Format
Abstract
A cDNA encoding β-mannanase was cloned from Aspergillus niger BCC4525 and expressed in Pichia pastoris KM71. The secreted enzyme hydrolyzed locust bean gum substrate with very high activity (1625 U/mL) and a relatively high kcat/Km (461 mg-1 s-1 mL). The enzyme is thermophilic and thermostable with an optimal temperature of 70°C and 40% retention of endo-β-1,4-mannanase activity after preincubation at 70°C. In addition, the enzyme exhibited broad pH stability with an optimal pH of 5.5. The recombinant enzyme hydrolyzes low-cost biomass, including palm kernel meal (PKM) and copra meal, to produce mannooligosaccharides, which is used as prebiotics to promote the growth of beneficial microflora in animals. An in vitro digestibility test simulating the gastrointestinal tract system of broilers suggested that the recombinant β-mannanase could effectively liberate reducing sugars from PKM-containing diet. These characteristics render this enzyme suitable for utilization as a feed additive to improve animal performance. © 2016 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
Keyword
Copra meal | Food and feed | Mannanase | Mannooligosaccharides | Palm kernel meal
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency; National Center for Genetic Engineering and Biotechnology
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Copyright
Rights
Publisher
Publication Source
Scopus