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Proteomic analysis of crocodile white blood cells reveals insights into the mechanism of the innate immune system
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Document Title
Proteomic analysis of crocodile white blood cells reveals insights into the mechanism of the innate immune system
Author
Tankrathok A. Mahong B. Roytrakul S. Daduang S. Temsiripong Y. Klaynongsruang S. Jangpromma N.
Affiliations
Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI) Faculty of Science Khon Kaen University Khon Kaen 40002 Thailand; Department of Biotechnology Faculty of Agricultural Technology Kalasin University Kalasin 46000 Thailand; Proteomics Laboratory Genome Technology Research Unit National Center for Genetic Engineering and Biotechnology Pathumthani 12120 Thailand; Division of Pharmacognosy and Toxicology Faculty of Pharmaceutical Sciences Khon Kaen University Khon Kaen 40002 Thailand; Sriracha Moda Co. Ltd. Chonburi Sriracha 20110 Thailand; Department of Biochemistry Faculty of Science Khon Kaen University Khon Kaen 40002 Thailand
Type
Article
Source Title
Heliyon
ISSN
24058440
Year
2024
Volume
10
Issue
2
Open Access
All Open Access Gold
Publisher
Elsevier Ltd
DOI
10.1016/j.heliyon.2024.e24583
Abstract
Crocodiles have a particularly powerful innate immune system because their blood contains high levels of antimicrobial peptides. They can survive injuries that would be fatal to other animals and they are rarely afflicted with diseases. To better understand the crocodile s innate immune response proteomic analysis was performed on the white blood cells (WBC) of an Aeromonas hydrophila-infected crocodile. Levels of WBC and red blood cells (RBC) rapidly increased within 1 h. In WBC there were 109 up-regulated differentially expressed proteins (DEP) that were up-regulated. Fifty-nine DEPs dramatically increased expression from 1 h after inoculation whereas 50 up-regulated DEPs rose after 24 h. The most abundant DEPs mainly had two biological functions 1) gene expression regulators for example zinc finger proteins and histone H1 family and 2) cell mechanical forces such as actin cytoskeleton proteins and microtubule-binding proteins. This finding illustrates the characteristic effective innate immune response mechanism of crocodiles that might occur via boosted transcription machinery proteins to accelerate cytoskeletal protein production for induction of phagocytosis along with the increment of trafficking proteins to transport essential molecules for combating pathogens. The findings of this study provide new insights into the mechanisms of the crocodile s innate immune system. ? 2024 The Authors
License
CC BY
Rights
Authors
Publication Source
WOS