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Proteomic Analysis of Serum and Urine of HIV-Monoinfected and HIV/HCV-Coinfected Patients Undergoing Long Term Treatment with Nevirapine
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Metadata
Document Title
Proteomic Analysis of Serum and Urine of HIV-Monoinfected and HIV/HCV-Coinfected Patients Undergoing Long Term Treatment with Nevirapine
Author
Wongtrakul J, Thongtan T, Roytrakul S, Kumrapich B, Janphen K, Praparattanapan J, Supparatpinyo K, Smith DR
Name from Authors Collection
Affiliations
Chiang Mai University; Chulalongkorn University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Chiang Mai University; Mahidol University
Type
Article
Source Title
DISEASE MARKERS
Year
2014
Volume
2014
Open Access
Green Published, Green Submitted, gold
Publisher
HINDAWI LTD
DOI
10.1155/2014/315824
Format
Abstract
Nevirapine (NVP) is an effective nonnucleoside reverse transcriptase inhibitor (NNRTI) of particular interest as it is often used in resource limited countries. However, one of the main concerns with the use of NVP is hepatotoxicity and elevation of liver enzymes as a consequence of highly active antiretroviral therapy (HAART) containing NVP is more often reported in HIV patients coinfected with hepatitis C virus than in HIV-monoinfected patients. To discover possible markers of NVP induced hepatotoxicity, serum and urine samples from twenty-five HIV or HIV/HCV patients, all of whom had received NVP continuously for at least four months, and healthy controls were subjected to in-solution or in-gel proteomic analysis. A total of 83 differentially regulated proteins consisted of 34 proteins identified in serum by in-solution analysis, 2 proteins identified from serum in a 2D gel electrophoresis analysis, and 47 proteins identified in urine in an in-solution analysis. Three proteins, namely, haptoglobin, Rho-related BTB domain containing protein 3, and death-associated protein kinase 3, were selected for further validation by Western blot analysis and results showed that haptoglobin has potential for further development as an additional marker of NVP induced hepatotoxicity.
Funding Sponsor
National Research Council of Thailand (NRCT); Chiang Mai University under the National Research Universities Initiative
License
CC-BY
Rights
Authors
Publication Source
WOS