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Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
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Document Title
Proteomics and Molecular Docking Analyses Reveal the Bio-Chemical and Molecular Mechanism Underlying the Hypolipidemic Activity of Nano-Liposomal Bioactive Peptides in 3T3-L1 Adipocytes
Author
Krobthong S. Yingchutrakul Y. Wongtrakoongate P. Chuntakaruk H. Rungrotmongkol T. Chaichana C. Mahatnirunkul T. Chomtong T. Choowongkomon K. Aonbangkhen C.
Affiliations
Center of Excellence in Natural Products Chemistry (CENP) Department of Chemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Center for Neuroscience Faculty of Science Mahidol University Bangkok 10400 Thailand; National Omics Center NSTDA Pathum Thani12120 Thailand; Department of Biochemistry Faculty of Science Mahidol University Bangkok 10400 Thailand; Center of Excellence in Biocatalyst and Sustainable Biotechnology Department of Biochemistry Faculty of Science Chulalongkorn University Bangkok 10330 Thailand; Program in Bioinformatics and Computational Biology Graduate School Chulalongkorn University Bangkok 10330 Thailand; Siriraj Center of Research Excellence for Diabetes and Obesity (SiCORE-DO) Faculty of Medicine Siriraj Hospital Mahidol University Bangkok 10700 Thailand; National Nanotechnology Center NSTDA Pathum Thani12120 Thailand; Department of Biochemistry Faculty of Science Kasetsart University Bangkok 10900 Thailand
Type
Article
Source Title
Foods
ISSN
23048158
Year
2023
Volume
12
Issue
4
Open Access
All Open Access Gold Green
Publisher
MDPI
DOI
10.3390/foods12040780
Abstract
Obesity is a global health concern. Physical activities and eating nutrient-rich functional foods can prevent obesity. In this study nano-liposomal encapsulated bioactive peptides (BPs) were developed to reduce cellular lipids. The peptide sequence NH2-PCGVPMLTVAEQAQ-CO2H was chemically synthesized. The limited membrane permeability of the BPs was improved by encapsulating the BPs with a nano-liposomal carrier which was produced by thin-layer formation. The nano-liposomal BPs had a diameter of ~157 nm and were monodispersed in solution. The encapsulation capacity was 61.2 ? 3.2%. The nano-liposomal BPs had no significant cytotoxicity on the tested cells keratinocytes fibroblasts and adipocytes. The in vitro hypolipidemic activity significantly promoted the breakdown of triglycerides (TGs). Lipid droplet staining was correlated with TG content. Proteomics analysis identified 2418 differentially expressed proteins. The nano-liposomal BPs affected various biochemical pathways beyond lipolysis. The nano-liposomal BP treatment decreased the fatty acid synthase expression by 17.41 ? 1.17%. HDOCK revealed that the BPs inhibited fatty acid synthase (FAS) at the thioesterase domain. The HDOCK score of the BPs was lower than that of orlistat a known obesity drug indicating stronger binding. Proteomics and molecular docking analyses confirmed that the nano-liposomal BPs were suitable for use in functional foods to prevent obesity. ? 2023 by the authors.
Keyword
adipocyte | Fatty acid synthase | glycerol | HDOCK | Lipolysis | Liposome | Nanoparticles | Orlistat
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS