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Regulation on antioxidant defense system in rice seedlings (Oryza sativa L. ssp. indica cv. ‘Pathumthani 1’) under salt stress by paclobutrazol foliar application
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Metadata
Document Title
Regulation on antioxidant defense system in rice seedlings (Oryza sativa L. ssp. indica cv. 'Pathumthani 1') under salt stress by paclobutrazol foliar application
Author
Khunpon B., Cha-Um S., Faiyue B., Uthaibutra J., Saengnil K.
Name from Authors Collection
Affiliations
Chiang Mai University, Faculty of Science, Department of Biology, Chiang Mai, 50200, Thailand; National Science and Technology Development Agency (NSTDA), National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Pahonyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Mahidol Wittayanusorn School, Department of Biology, Salaya, Phuttamonthon, Nakhon Pathom, 73170, Thailand; Chiang Mai University, Faculty of Science, Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Chiang Mai, 50200, Thailand
Type
Article
Source Title
Notulae Botanicae Horti Agrobotanici Cluj-Napoca
ISSN
0255965X
Year
2019
Volume
47
Issue
2
Page
368-377
Open Access
Gold
Publisher
Academic Press
DOI
10.15835/nbha47111282
Format
Abstract
The present study investigated the effect of paclobutrazol (PBZ) foliar application on oxidative metabolism in salt-stressed rice (Oryza sativa L. cv. 'Pathumthani 1'; PTT1) seedlings. Fourteen-days-old rice seedlings, grown in the pots were pretreated with 15 mg L-1 paclobutrazol supplied as foliar spray. One week after pretreatment, the rice seedlings were exposed to salt stress (150 mM NaCl) for 12 days. It was observed that salinity enhanced the production of reactive oxygen species (ROS), including superoxide radical (O2 •-), hydrogen peroxide (H2O2) and hydroxyl radical (OH•). It also increased reactive oxygen speciesassociated oxidative damage, measured in terms of lipoxygenase activity, conjugated dienes, malondialdehyde content and relative electrolyte leakage. Increase in these parameters was associated with the decrease in the activity of enzymatic antioxidants [superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT)] and the levels of non-enzymatic antioxidants [ascorbic acid (AsA), total glutathione and α-tocopherol contents). Pretreatment of seedlings with paclobutrazol significantly lowered reactive oxygen species accumulation and membrane damage (p < 0.05), which can be correlated with the increased antioxidant activity (both enzymatic and non-enzymatic traits) under salt stress. The study concluded that paclobutrazol-treatment up-regulates the antioxidant defense system and recuperates the salt-induced oxidative damage in 'Pathumthani 1' rice seedlings under salt stress. © Not Bot Horti Agrobo, 2019.
Funding Sponsor
National Science and Technology Development Agency; Thailand Graduate Institute of Science and Technology; Graduate School, Chiang Mai University
License
CC BY
Rights
Author
Publication Source
Scopus
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