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Role of CD61(+) cells in thrombocytopenia of dengue patients
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Document Title
Role of CD61(+) cells in thrombocytopenia of dengue patients
Author
Noisakran S, Onlamoon N, Pattanapanyasat K, Hsiao HM, Songprakhon P, Angkasekwinai N, Chokephaibulkit K, Villinger F, Ansari AA, Perng GC
Name from Authors Collection
Affiliations
Emory University; National Science & Technology Development Agency - Thailand; National Center Genetic Engineering & Biotechnology (BIOTEC); Mahidol University; Mahidol University; Mahidol University; Mahidol University; Emory University
Type
Article
Source Title
INTERNATIONAL JOURNAL OF HEMATOLOGY
Year
2012
Volume
96
Issue
5
Page
600-610
Open Access
Green Accepted
Publisher
SPRINGER JAPAN KK
DOI
10.1007/s12185-012-1175-x
Format
Abstract
Although hematological disorders with salient features of thrombocytopenia have been well documented in dengue patients, the role of CD61-expressing platelets and the megakaryocytic cell lineage in the pathogenesis of dengue virus (DENV) infection remains largely unexplored. A prospective observational study was performed using blood samples and PBMCs from dengue-confirmed patients, as well as from rhesus monkeys (RM) experimentally infected with DENV. Immunohistochemical staining and FACS techniques were applied to evaluate the frequencies of CD61(+) cells that contained DENV antigen. Highly enriched population of CD61(+) cells was also isolated from acute DENV-infected RM and assayed for DENV RNA by quantitative RT-PCR. Results revealed that DENV antigen was found in small vesicles of varying size, and more frequently in anucleated cells associated with platelets in dengue patients. The DENV antigen-containing cells were CD61(+) and appeared to share characteristics of megakaryocytes. Kinetic profiles of CD61(+) cells from DENV-infected RM revealed a transient increase in CD61(+)CD62P(+) cells early after DENV infection. DENV RNA in a highly enriched population of CD61(+) cells from the infected RM was observed during acute stage. Our results indicate that virus containing CD61(+) cells may be directly linked to the platelet dysfunction and low platelet count characteristics of dengue patients.
Keyword
Dengue | DF | DHF | Fever | Megakaryocytes | Thrombocytopenia | Viremia
Industrial Classification
Knowledge Taxonomy Level 3
Funding Sponsor
Emory SOM start-up fund; Thailand Research Fund for Senior Research Scholar; Robert E. Shop International Fellowship; U19 Pilot Project Funds [U19 AI057266 (RFA-AI-02-042)]; NIH/SERCEB; Emory URC; NCRR p51 [DRR000165]; NATIONAL CENTER FOR RESEARCH RESOURCES [P51RR000165] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U19AI057266] Funding Source: NIH RePORTER; OFFICE OF THE DIRECTOR, NATIONAL INSTITUTES OF HEALTH [R24OD010947] Funding Source: NIH RePORTER
License
Copyright
Rights
The Japanese Society of Hematology 2012
Publication Source
WOS