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Shrimp injection with dsRNA targeting the microsporidian EHP polar tube protein reduces internal and external parasite amplification
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Document Title
Shrimp injection with dsRNA targeting the microsporidian EHP polar tube protein reduces internal and external parasite amplification
Author
Yuanlae S. Prasartset T. Reamtong O. Munkongwongsiri N. Panphloi M. Preechakul T. Suebsing R. Thitamadee S. Prachumwat A. Itsathitphaisarn O. Taengchaiyaphum S. Kasamechotchung C.
Affiliations
Department of Biochemistry Faculty of Science Mahidol University Rama VI Rd. Bangkok 10400 Thailand; Center for Excellence in Shrimp Molecular Biology and Biotechnology (Centex Shrimp) Faculty of Science Mahidol University Rama VI Rd. Bangkok 10400 Thailand; Department of Biotechnology Faculty of Science Mahidol University Rama VI Rd. Bangkok 10400 Thailand; Department of Molecular Tropical Medicine and Genetics Faculty of Tropical Medicine Mahidol University Bangkok 10400 Thailand; Aquatic Animal Health Research Team (AQHT) Integrative Aquaculture Biotechnology Research Group National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Yothi Office Rama VI Rd. Bangkok 10400 Thailand; Enzyme Technology Laboratory National Center for Genetic Engineering and Biotechnology (BIOTEC) 113 Thailand Science Park Phaholyothin Road Khlong Luang Pathumthani 12120 Thailand; Analytical Sciences and National Doping Test Institute Mahidol University Rama VI Rd. Bangkok 10400 Thailand; Department of Fisheries Faculty of Agriculture and Natural Resources Rajamangala University of Technology Tawan-ok Chonburi 20110 Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2024
Volume
14
Issue
1
Open Access
All Open Access Gold Green
Publisher
Nature Research
DOI
10.1038/s41598-024-55400-2
Abstract
The microsporidian Enterocytozoon hepatopenaei (EHP) is a major threat to shrimp health worldwide. Severe EHP infections in shrimp cause growth retardation and increase susceptibility to opportunistic infections. EHP produces spores with a chitin wall that enables them to survive prolonged environmental exposure. Previous studies showed that polar tube extrusion is a prerequisite for EHP infection such that inhibiting extrusion should prevent infection. Using a proteomic approach polar tube protein 2 of EHP (EhPTP2) was found abundantly in protein extracts obtained from extruded spores. Using an immunofluorescent antibody against EhPTP2 for immunohistochemistry extruded spores were found in the shrimp hepatopancreas (HP) and intestine but not in the stomach. We hypothesized that presence of EhPTP2 might be required for successful EHP spore extrusion. To test this hypothesis we injected EhPTP2-specific double-stranded RNA (dsRNA) and found that it significantly diminished EHP copy numbers in infected shrimp. This indicated reduced amplification of EHP-infected cells in the HP by spores released from previously infected cells. In addition injection of the dsRNA into EHP-infected shrimp prior to their use in cohabitation with na?ve shrimp significantly (p < 0.05) reduced the rate of EHP transmission to na?ve shrimp. The results revealed that EhPTP2 plays a crucial role in the life cycle of EHP and that dsRNA targeting EHP mRNA can effectively reach the parasite developing in host cells. This approach is a model for future investigations to identify critical genes for EHP survival and spread as potential targets for preventative and therapeutic measures in shrimp. ? The Author(s) 2024.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus