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Species identification of non-hybrid and hybrid Pangasiid catfish using polymerase chain reaction-restriction fragment length polymorphism
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Metadata
Document Title
Species identification of non-hybrid and hybrid Pangasiid catfish using polymerase chain reaction-restriction fragment length polymorphism
Author
Sriphairoj K., Na-Nakorn U., Klinbunga S.
Name from Authors Collection
Affiliations
Faculty of Natural Resources and Agro-Industry, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusSakon Nakhon, Thailand; Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand; Aquatic Molecular Genetics and Biotechnology Laboratory, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Thailand; Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand
Type
Article
Source Title
Agriculture and Natural Resources
ISSN
24681458
Year
2018
Volume
52
Issue
1
Page
99-105
Open Access
Hybrid Gold
Publisher
Elsevier B.V.
DOI
10.1016/j.anres.2018.05.014
Abstract
Species-diagnostic molecular markers are essential for the identification of species groups possessing overlapping morphological characters. They also play an important role in preventing the supply of incorrect species for the food industry and in verifying the species origins of various forms of products. Molecular genetic markers were developed for the identification of six Pangasiid species—Pangasianodon gigas, Pangasianodon hypophthalmus, Pangasius bocourti, Pangasius conchophilus, Pangasius larnaudii and Pangasius sanitwongsei—based on restriction analysis of mitochondrial 16S ribosomal (r) DNA with Tsp 509I, Hinc II and Mfe I and nuclear immunoglobulin M heavy chain constant region (IgM-H) with Taq I and Pst I. Six non-overlapping composite restriction fragment length polymorphism (RFLP) patterns: AAB (AA) (AA), ACB(AA) (BB), BBB(BB) (BB), CCB(BB) (BB), DCA (BB) (CC) and DCB(−) (−) were observed across the respective species. Larvae of P. gigas, P. hypophthalmus, P. bocourti, P. larnaudii and P. sanitwongsei could be unambiguously differentiated using polymerase chain reaction (PCR)-RFLP analysis of 16S rDNA. In addition, detection of P. hypophthalmus × P. gigas, P. hypophthalmus × P. bocourti and P. hypophthalmus × P. larnaudii hybrids could be carried out using PCR-RFLP analysis of IgM-H. © 2018 Kasetsart University
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
Office of the Higher Education Commission; Thailand Research Fund
License
CC BY-NC-ND
Rights
Kasetsart University
Publication Source
Scopus