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Species identification of the tropical abalone (Haliotis asinina, Haliotis ovina, and Haliotis varia) in Thailand using RAPD and SCAR markers
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Document Title
Species identification of the tropical abalone (Haliotis asinina, Haliotis ovina, and Haliotis varia) in Thailand using RAPD and SCAR markers
Author
Klinbunga S., Amparyup P., Leelatanawit R., Tassanakajon A., Hirono I., Aoki T., Jarayabhand P., Menasveta P.
Name from Authors Collection
Affiliations
Marine Biotechnology Research Unit, Natl. Ctr. Genetic Eng. and Biotech., Natl. Sci. and Technol. Devmt. Agy., 113 Paholyothin Rd., Klong 1, Klong Luang, Pathumthani 12120, Thailand; Program of Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Laboratory of Genome Science, Grad. Sch. of Mar. Sci. and Technol., Tokyo Univ. Mar. Sci. and Technol., Konan 4-5-7, Minato-ku, Tokyo 108-8477, Japan; Aquatic Resources Research Institute, Chulalongkorn University, Bangkok, 10330, Thailand
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2004
Volume
37
Issue
2
Page
213-222
Open Access
Bronze
Publisher
Springer Verlag
DOI
10.5483/bmbrep.2004.37.2.213
Abstract
A randomly amplified polymorphic DNA (RAPD) analysis was used to identify the species- and population-specific markers of abalone; Haliotis asinina, H. ovina, and H. varia in Thai waters. Fifteen species-specific and six population-specific RAPD markers were identified. In addition, an 1650 bp band (UBC195) that was restricted to H. ovina from the Gulf of Thailand (east) was also found. All of the specific RAPD markers were cloned and sequenced. Twenty pairs of primers were designed and specificity-tested (N = 12 and 4 for target and non-target species, respectively). Seven primer pairs (CUHA1, 2, 4, 11, 12, 13, and 14) were specifically amplified by H. asinina DNA, whereas a single pair of primers showed specificity with H. ovina (CUHO3) and H. varia (CUHV1), respectively. Four primer pairs, including CUHA2, CUHA12, CUHO3, and CUHV1, were further examined against 216 individuals of abalone (N = 111, 73, and 32, respectively). Results indicated the species-specific nature of all of them, except CUHO3, with the sensitivity of detection of 100 pg and 20 pg of the target DNA template for CUHA2 and CUHA12 and CUHV1, respectively. The species-origin of the frozen, ethanol-preserved, dried, and boiled H. asinina specimens could also be successfully identified by CUHA2.
Keyword
Abalone | PCR | RAPD | SCARs | Species-specific markers
License
CC BY-NC
Rights
Author
Publication Source
Scopus