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Streptomyces爎ugosispiralis sp. nov. a Novel Actinobacterium Isolated from Peat Swamp Forest Soil That Produces Ansamycin Derivatives and Nocardamines
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Document Title
Streptomyces爎ugosispiralis sp. nov. a Novel Actinobacterium Isolated from Peat Swamp Forest Soil That Produces Ansamycin Derivatives and Nocardamines
Author
Weeraphan T. Supong K. Sripreechasak P. Jutakanoke R. Kowinthanaphat S. Tanasupawat S. Pittayakhajonwut P. Phongsopitanun W.
Affiliations
Department of Biochemistry and Microbiology Faculty of Pharmaceutical Sciences Chulalongkorn University Bangkok 10330 Thailand; Department of Applied Science and Biotechnology Faculty of Agro-Industrial Technology Rajamangala University of Technology Tawan-ok Chantaburi 22210 Thailand; Office of Educational Affairs Faculty of Science Burapha University Chonburi 20131 Thailand; Department of Microbiology and Parasitology Faculty of Medical Science Naresuan University Phitsanulok 65000 Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Thailand Science Park Pathum Thani12120 Thailand; Natural Products and Nanoparticles Research Units (NP2) Chulalongkorn University Bangkok 10330 Thailand
Type
Article
Source Title
Antibiotics
ISSN
20796382
Year
2023
Volume
12
Issue
9
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/antibiotics12091467
Abstract
Actinomycetes especially the genus Streptomyces are one of the most promising sources of bioactive natural products. In this study a novel Streptomyces strain RCU-064T was isolated from a soil sample collected from a peat swamp forest in Thailand. Strain RCU-064T showed the highest 16S rRNA gene sequence similarity (99.06%) with Streptomyces爉alaysiensis NBRC 16446T. Based on a polyphasic approach strain RCU-064T represents a novel species of the genus Streptomyces for which the name Streptomyces爎ugosispiralis sp. nov. is proposed. The chemical isolation of the crude ethyl acetate extracts of the strain led to the isolation of six compounds: (1) geldanamycin (2) 17-O-demethylgeldanamycin (3) reblastatin (4) 17-demethoxyreblastatin (5) nocardamine and (6) dehydroxynocardamine. These compounds were evaluated for their biological activities. All compounds showed no antimicrobial activity against tested microorganisms used in this study. Compounds (1)�(4) displayed cytotoxic activity against the NCI-H187 cell line with IC50 values ranging from 0.045�250 ?g/mL. Cytotoxicity against the MCF-7 cell line was found in compounds (1) and (3) with IC50 values of 3.51 and 1.27 ?g/mL respectively. Compounds (5) and (6) exhibited cytotoxicity only against Vero cells (IC50 of 16.57 ?g/mL) and NCI-H187 cells (IC50 of 13.96 ?g/mL) respectively. These results indicate that peat swamp forest soil remains a promising reservoir of novel actinomycetes capable of producing bioactive natural products. ? 2023 by the authors.
Keyword
Actinomycetota | geldanamycin | Natural products | nocardamines | Polyphasic taxonomy | Streptomyces
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS