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Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon
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Document Title
Suppression subtractive hybridization (SSH) for isolation and characterization of genes related to testicular development in the giant tiger shrimp Penaeus monodon
Author
Leelatanawit R., Klinbunga S., Aoki T., Hirono I., Valyasevi R., Menasveta P.
Name from Authors Collection
Affiliations
Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence for Marine Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Paholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand; Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Tokyo 108-8477, Japan
Type
Article
Source Title
Journal of Biochemistry and Molecular Biology
ISSN
12258687
Year
2008
Volume
41
Issue
11
Page
796-802
Open Access
Gold, Green
Publisher
The Biochemical Society of the Republic of Korea
DOI
10.5483/bmbrep.2008.41.11.796
Abstract
Suppression subtractive hybridization (SSH) cDNA libraries of the giant tiger shrimp, Penaeus monodon, were constructed. In total, 178 and 187 clones from the forward and reverse SSH libraries, respectively, of P. monodon were unidirectionally sequenced. From these, 37.1% and 53.5% Expressed Sequence Tags (ESTs) significantly matched known genes (E-value < 1e-04). Three isoforms of P. monodon progestin membrane receptor component 1: PM-PGMRC1-s (1980 bp), PM-PGMRC1- m (2848 bp), and PM-PGMRC1-l (2971 bp), with an identical ORF of 573 bp corresponding to a deduced polypeptide of 190 amino acids, were successfully identified by RACE-PCR. Interestingly, PMPGMRC1 showed a greater expression level in testes of juvenile than broodstock P. monodon (P < 0.05). Dopamine administration (10-6 mol/shrimp) resulted in up-regulation of PMPGMRC1 in testes of juveniles at 3 hrs post treatment (P < 0.05), but had no effect on PM-Dmc1 (P > 0.05).
Keyword
Dmc1 | Inx2 | PGMRC1 | Real-time PCR | SSH
License
CC BY-NC
Rights
Author
Publication Source
Scopus