-
Synthesis of Carbohydrate Capped Silicon Nanoparticles and their Reduced Cytotoxicity, In Vivo Toxicity, and Cellular Uptake
- Back
Document Title
Synthesis of Carbohydrate Capped Silicon Nanoparticles and their Reduced Cytotoxicity, In Vivo Toxicity, and Cellular Uptake
Author
Ahire JH, Behray M, Webster CA, Wang Q, Sherwood V, Saengkrit N, Ruktanonchai U, Woramongkolchai N, Chao YM
Name from Authors Collection
Affiliations
University of East Anglia; University of East Anglia; National Science & Technology Development Agency - Thailand; National Nanotechnology Center (NANOTEC)
Type
Article
Source Title
ADVANCED HEALTHCARE MATERIALS
ISSN
2192-2640
Year
2015
Volume
4
Issue
1
Open Access
Green Accepted
Publisher
WILEY
DOI
10.1002/adhm.201500298
Format
Abstract
The development of smart targeted nanoparticles (NPs) that can identify and deliver drugs at a sustained rate directly to cancer cells may provide better efficacy and lower toxicity for treating primary and advanced metastatic tumors. Obtaining knowledge of the diseases at the molecular level can facilitate the identification of biological targets. In particular, carbohydrate-mediated molecular recognitions using nano-vehicles are likely to increasingly affect cancer treatment methods, opening a new area in biomedical applications. Here, silicon NPs (SiNPs) capped with carbohydrates including galactose, glucose, mannose, and lactose are successfully synthesized from amine terminated SiNPs. The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] analysis shows an extensive reduction in toxicity of SiNPs by functionalizing with carbohydrate moiety both in vitro and in vivo. Cellular uptake is investigated with flow cytometry and confocal fluorescence microscope. The results show the carbohydrate capped SiNPs can be internalized in the cells within 24 h of incubation, and can be taken up more readily by cancer cells than non-cancerous cells. Moreover, these results reinforce the use of carbohydrates for the internalization of a variety of similar compounds into cancer cells.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
University of East Anglia; Royal Society; British Skin Foundation; CRUK; Engineering and Physical Sciences Research Council [1212695] Funding Source: researchfish
License
Copyright
Rights
Publisher
Publication Source
WOS