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Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
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Metadata
Document Title
Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
Author
Chansriniyom C.,Nooin R.,Nuengchamnong N.,Wongwanakul R.,Petpiroon N.,Srinuanchai W.,Chantarasuwan B.,Pitchakarn P.,Temviriyanukul P.,Nuchuchua O.
Name from Authors Collection
Scopus Author ID
55875176500
Affiliations
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Natural Products and Nanoparticles Research Unit, Chulalongkorn University, Bangkok, Thailand; Nano Agricultural Chemistry and Processing Research Team, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand; Science Laboratory Center, Faculty of Science, Naresuan University, Phitsanulok, Thailand; Nano Environmental and Health Safety Research Team, Advanced Nanocharacterization and Safety Research Group, National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathumthani, Thailand; National Science Museum, Technopolish, Klong 5, Klong Luang, Pathumthani, Thailand; Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Food and Nutrition Academic and Research Cluster, Institute of Nutrition, Mahidol University, Salaya, Nakhon Pathom, Phuttamonthon, Thailand
Type
Article
Source Title
Scientific Reports
ISSN
20452322
Year
2021
Volume
11
Issue
1
Open Access
All Open Access, Gold, Green
Publisher
Nature Research
DOI
10.1038/s41598-021-96261-3
Abstract
Since 2006, Ficus dubia has been reported as a new Ficus species in Thailand. As per our recent report, the red-brown aqueous extract of F. dubia sap (FDS) has been determined to strongly exhibit in vitro anti-radicals. However, the phytochemicals in the FDS extract related to health-promoting antioxidation have not been explored. Thus, in this study, we aimed to investigate the chemical components of the F. dubia sap extract by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC–ESI–MS/QTOF-MS) and its potential use in cosmetics in terms of cellular antioxidation on keratinocytes (HaCaT), phototoxicity, and irritation on 3D skin cell models following standard tests suggested by the Organization for Economic Cooperation and Development (OECD). It was found that the sap extract was composed of quinic acid and caffeoyl derivatives (e.g., syringoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, and dimeric forms of caffeoylquinic acids). The extract has significantly exhibited antioxidant activity against H2O2-induced oxidative stress in HaCaT cells. The cellular antioxidative effect of the FDS extract was remarkably dependent on the presence of 3- and 4-O-caffeoylquinic acid in the extract. Furthermore, the FDS extract showed negative results on skin phototoxicity and irritation. Overall, the results reveal that the FDS extract could be developed as a new antioxidant candidate for a skin healthcare product. © 2021, The Author(s).
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Science and Technology Development Agency
License
CC BY
Rights
Publisher
Publication Source
Scopus