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Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats
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Document Title
Targeted proteomic analysis reveals that crocodile oil from Crocodylus siamensis may enhance hepatic energy metabolism in rats
Author
Fungfuang W. Srisuksai K. Santativongchai P. Charoenlappanit S. Phaonakrop N. Roytrakul S. Tulayakul P. Parunyakul K.
Affiliations
Kasetsart University Research and Development Institute Kasetsart University Ngamwongwan Road Chatuchak Bangkok 10900 Thailand; Department of Zoology Faculty of Science Kasetsart University Ngamwongwan Road Chatuchak Bangkok 10900 Thailand; Bio-Veterinary Science (International Program) Faculty of Veterinary Medicine Kasetsart University Ngamwongwan Road Chatuchak Bangkok 10900 Thailand; Functional Ingredients and Food Innovation Research Group National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency Phahonyothin Road Khlong Nueang Pathum Thani Khlong Luang 12120 Thailand; Department of Veterinary Public Health Faculty of Veterinary Medicine Kasetsart University Malaiman Road Kamphaeng Saen Nakhon Pathom 73140 Thailand
Type
Article
Source Title
Experimental Animals
ISSN
13411357
Year
2023
Volume
72
Issue
4
Page
425-438
Open Access
All Open Access Gold Green
Publisher
International Press Editing Centre Incorporation
DOI
10.1538/expanim.23-0009
Abstract
The liver is a key organ governing body energy metabolism. Dietary fats influence energy metabolism and mitochondrial functioning. Crocodile oil (CO) is rich in mono-and polyunsaturated fatty acids that contain natural anti-inflammatory and healing properties. Our study examined how CO affects the expressions of liver proteins involved in energy metabolism in rats. Twenty-one male Sprague Dawley rats were divided into three groups and underwent oral gavage with 3 ml/kg of sterile water (N group) CO (CO group) or palm oil (PO group) for 7 weeks. Body weight energy intake liver weight liver indexes blood lipid profiles and liver-energy intermediates were measured. The liver proteome was analyzed using shotgun proteomics and the functions and network interactions of several candidate proteins were predicted using the STITCH v.5.0 software. Body weights energy intake liver contents and lipid profiles did not differ between the groups. However hepatic oxaloacetate and malate levels were significantly higher in the CO group than in the PO group. Targeted proteomics reveals that 22 out of 1 790 unique proteins in the CO group were involved in energy-generating pathways including the tricarboxylic acid cycle and oxidative phosphorylation (OXPHOS) and were correlated with the AMP-activated protein kinase signaling pathway. Cluster analysis of 59 differentially expressed proteins showed that OXPHOS-associated proteins were upregulated in the CO group and that three glycolytic metabolism-related proteins were downregulated in the CO group. CO may enhance hepatic energy metabolism by regulating the expressions of energy expenditure-related proteins. ? 2023 Japanese Association for Laboratory Animal Science.
Keyword
Crocodile oil | Energy metabolism | liver | Proteomics | Rat
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY-NC-ND
Rights
Japanese Assocition for Laborator Animal Science
Publication Source
WOS