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The Toxicological Assessment of Anoectochilus burmannicus Ethanolic-Extract-Synthesized Selenium Nanoparticles Using Cell Culture Bacteria and Drosophila melanogaster as Suitable Models
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Document Title
The Toxicological Assessment of Anoectochilus burmannicus Ethanolic-Extract-Synthesized Selenium Nanoparticles Using Cell Culture Bacteria and Drosophila melanogaster as Suitable Models
Author
Buacheen P. Karinchai J. Inthachat W. Butkinaree C. Jankam C. Wongnoppavich A. Imsumran A. Chewonarin T. Pimpha N. Temviriyanukul P. Pitchakarn P.
Affiliations
PhD Program in Biochemistry Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand; Department of Biochemistry Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand; Food and Nutrition Academic and Research Cluster Institute of Nutrition Mahidol University Nakhon Pathom 73170 Thailand; National Omics Center National Science and Technology Development Agency Pathum Thani 12120 Thailand; National Nanotechnology Center National Science and Technology Development Agency Thailand Science Park Pathum Thani 12120 Thailand
Type
Article
Source Title
Nanomaterials
ISSN
20794991
Year
2023
Volume
13
Issue
20
Open Access
All Open Access Gold Green
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
DOI
10.3390/nano13202804
Abstract
Selenium nanoparticles (SeNPs) are worthy of attention and development for nutritional supplementation due to their health benefits in both animals and humans with low toxicity improved bioavailability and controlled release being greater than the Se inorganic and organic forms. Our previous study reported that Anoectochilus burmannicus extract (ABE)-synthesized SeNPs (ABE-SeNPs) exerted antioxidant and anti-inflammatory activities. Furthermore ABE could stabilize and preserve the biological activities of SeNPs. To promote the ABE-SeNPs as supplementary and functional foods it was necessary to carry out a safety assessment. Cytotoxicity testing showed that SeNPs and ABE-SeNPs were harmless with no killing effect on Caco2 (intestinal epithelial cells) MRC-5 (lung fibroblasts) HEK293 (kidney cells) LX-2 (hepatic stellate cells) and 3T3-L1 (adipocytes) and were not toxic to isolated human PBMCs and RBCs. Genotoxicity assessments found that SeNPs and ABE-SeNPs did not induce mutations in Salmonella typhimurium TA98 and TA100 (Ames test) as well as in Drosophila melanogaster (somatic mutation and recombination test). Noticeably ABE-SeNPs inhibited mutation in TA98 and TA100 induced by AF-2 and in Drosophila induced by urethane ethyl methanesulfonate and mitomycin c suggesting their anti-mutagenicity ability. This study provides data that support the safety and anti-genotoxicity properties of ABE-SeNPs for the further development of SeNPs-based food supplements. ? 2023 by the authors.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
Scopus