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The vitamin D receptor agonist EB1089 can exert its antiviral activity independently of the vitamin D receptor
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Document Title
The vitamin D receptor agonist EB1089 can exert its antiviral activity independently of the vitamin D receptor
Author
Jaratsittisin J. Sornjai W. Chailangkarn T. Jongkaewwattana A. Smith D.R.
Affiliations
Institute of Molecular Biosciences Mahidol University Salaya Thailand; Virology and Cell Technology Research Team National Center of Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Pathum Thani Thailand
Type
Article
Source Title
PLoS ONE
ISSN
19326203
Year
2023
Volume
18
Issue
10-�.�.
Open Access
All Open Access Gold Green
Publisher
Public Library of Science
DOI
10.1371/journal.pone.0293010
Abstract
Vitamin D has been shown to have antiviral activity in a number of different systems. However few studies have investigated whether the antiviral activity is exerted through the vitamin D receptor (VDR). In this study we investigated whether the antiviral activity of a vitamin D receptor agonist (EB1089) towards dengue virus (DENV) was modulated by VDR. To undertake this VDR was successively overexpressed knocked down and retargeted through mutation of the nuclear localization signal. In no case was an effect seen on the level of the antiviral activity induced by EB1089 strongly indicating that the antiviral activity of EB1089 is not exerted through VDR. To further explore the antiviral activity of EB1089 in a more biologically relevant system human neural progenitor cells were differentiated from induced pluripotent stem cells and infected with Zika virus (ZIKV). EB1089 exerted a significant antiviral effect reducing virus titers by some 2Log10. In support of the results seen with DENV no expression of VDR at the protein level was observed. Collectively these results show that the vitamin D receptor agonist EB1089 exerts its antiviral activity independently of VDR. Copyright: ? 2023 Jaratsittisin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License which permits unrestricted use distribution and reproduction in any medium provided the original author and source are credited.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Authors
Publication Source
WOS