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Thermal impacts on transcriptome of Pectoralis major muscle collected from commercial broilers Thai native chickens and its crossbreeds
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Document Title
Thermal impacts on transcriptome of Pectoralis major muscle collected from commercial broilers Thai native chickens and its crossbreeds
Author
Malila Y. Uengwetwanit T. Sanpinit P. Songyou W. Srimarut Y. Kunhareang S.
Affiliations
National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Khlong Nueng Khlong Luang Pathum Thani 12120 Thailand; Department of Animal Science Faculty of Agriculture Khon Kaen University Khon Kaen 40002 Thailand
Type
Article
Source Title
Animal Bioscience
ISSN
27650189
Year
2024
Volume
37
Issue
1
Page
61-73
Open Access
All Open Access Gold Green
Publisher
Asian-Australasian Association of Animal Production Societies
DOI
10.5713/ab.23.0195
Abstract
Objective: The main objective of this study was to define molecular mechanisms associated with thermal stress responses of chickens from commercial broilers (BR Ross 308) Thai native chickens (NT) and crossbreeds between BR?NT (H75). Methods: Twenty days before reaching specific market age chickens from each breed were divided into control and thermal-stressed groups. The stressed groups were exposed to a cyclic thermal challenge (35?C?1?C for 6 h followed by 26?C?1?C for 18 h) for 20 days. Control group was raised under a constant temperature of 26?C?1?C. Pectoralis major (n = 4) from each group was collected for transcriptome analysis using HiSeq Illumina and analysis of glycogen and lactate. Gene expression patterns between control and thermal-stressed groups were compared within the same breeds. Results: Differentially expressed transcripts of 65 59 and 246 transcripts for BR NT and H75 respectively were revealed by RNA-Seq and recognized by Kyoto encyclopedia of genes and genomes database. Pathway analysis underlined altered glucose homeostasis and protein metabolisms in all breeds. The signals centered around phosphatidylinositol 3-kinase (PI3K)/Akt signaling focal adhesion and MAPK signaling in all breeds with slight differences in molecular signal transduction patterns among the breeds. An extensive apoptosis was underlined for BR. Roles of AMPK MAPK signaling and regulation of actin cytoskeleton in adaptive response were suggested for H75 and NT chickens. Lower glycogen content was observed in the breast muscles of BR and NT (p<0.01) compared to their control counterparts. Only BR muscle exhibited increased lactate (p<0.01) upon exposure to the stress. Conclusion: The results provided a better comprehension regarding the associated biological pathways in response to the cyclic thermal stress in each breed and in chickens with different growth rates. Copyright ? 2024 by Animal Bioscience.
Keyword
Chickens | Gene Expression | Native Breed | RNA-seq | thermal stress
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
License
CC BY
Rights
Animal Bioscience
Publication Source
WOS