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Transcriptional analysis of the Streptococcus pyogenes salivaricin locus
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Metadata
Document Title
Transcriptional analysis of the Streptococcus pyogenes salivaricin locus
Author
Namprachan-Frantz P., Rowe H.M., Runft D.L., Neely M.N.
Name from Authors Collection
Affiliations
Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, MI, United States; Phanramphoei Namprachan-Frantz, National Science and Technology Development Agency, National Center for Genetic Engineering and Biotechnology, Rangsit, Pathum Thani, Thailand
Type
Article
Source Title
Journal of Bacteriology
ISSN
00219193
Year
2014
Volume
196
Issue
3
Page
604-613
Open Access
All Open Access, Bronze, Green
DOI
10.1128/JB.01009-13
Format
Abstract
The sal lantibiotic locus plays an important role in the virulence of Streptococcus pyogenes. Our transcriptional analysis of the sal locus provides new information on the complex regulation of this operon. Transcription of the operon is regulated by a promoter upstream of the operon and by a second internal promoter upstream of the salKRZ genes. Here we identify the location of the internal promoter and provide information on how this promoter is autoregulated by proteins within the locus. We determined by primer extension that the salKR promoter is located within the salY gene and identified several regulatory regions important for expression. The higher activity of the promoter in a salKR deletion strain indicates a role in repression by the SalR response regulator. Further, this promoter had higher activity in a salA deletion strain, implicating corepression or a signaling role for the SalA peptide. Finally, we demonstrate that this promoter can be controlled by host factors. Analysis of transcriptional regulation of this locus provides a better understanding of the function of the sal locus in S. pyogenes pathogenesis. © 2014, American Society for Microbiology.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
National Institute of Allergy and Infectious Diseases
License
N/A
Rights
N/A
Publication Source
Scopus