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Validation of PfSNP-LAMP-lateral flow dipstick for detection of single nucleotide polymorphism associated with pyrimethamine resistance in plasmodium falciparum
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Metadata
Document Title
Validation of PfSNP-LAMP-lateral flow dipstick for detection of single nucleotide polymorphism associated with pyrimethamine resistance in plasmodium falciparum
Author
Yongkiettrakul S., Kolié F.R., Kongkasuriyachai D., Sattabongkot J., Nguitragool W., Nawattanapaibool N., Suansomjit C., Warit S., Kangwanrangsan N., Buates S.
Name from Authors Collection
Scopus Author ID
6506219723
Affiliations
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand; Department of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand; Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand
Type
Article
Source Title
Diagnostics
ISSN
20754418
Year
2020
Volume
10
Issue
11
Open Access
Gold
Publisher
MDPI AG
DOI
10.3390/diagnostics10110948
Format
Abstract
The loop-mediated isothermal amplification coupled with lateral flow dipstick (Pf SNP-LAMP-LFD) was recently developed to detect single nucleotide polymorphism (AAT → ATT), corresponding to substitution of asparagine to isoleucine at amino acid position 51 in the P. falciparum dhfr-ts gene associated with antifolate resistance. In this present study, the Pf SNP-LAMP-LFD was validated on 128 clinical malaria samples of broad ranged parasite densities (10 to 87,634 parasites per microliter of blood). The results showed 100% accuracy for the detection of single nucleotide polymorphism for N51I mutation. Indeed, the high prevalence of N51I in the Pfdhfr-ts gene detected in the clinical samples is in line with reports of widespread antifolate resistant P. falciparum in Thailand. The relationship between enzyme choice and reaction time was observed to have an effect on Pf SNP-LAMP-LFD specificity; however, the method yielded consistent results once the conditions have been optimized. The results demonstrate that Pf SNP-LAMP-LFD is a simple method with sufficient sensitivity and specificity to be deployed in routine surveillance of antifolate resistance molecular marker and inform antimalarial management policy. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. T.
Funding Sponsor
Faculty of Science, Mahidol University
License
CC BY
Rights
Author
Publication Source
Scopus
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