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Valorisation of sawdust-based spent mushroom substrate for sustainable xylooligosaccharides production using low-cost crude xylanases from Aspergillus flavus KUB2
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Document Title
Valorisation of sawdust-based spent mushroom substrate for sustainable xylooligosaccharides production using low-cost crude xylanases from Aspergillus flavus KUB2
Author
Supmeeprom S. Thammasittirong A. Jeennor S. Sopalun K. Thammasittirong S.N.-R.
Affiliations
Department of Science and Bioinnovation Faculty of Liberal Arts and Science Kasetsart University Nakhon Pathom Thailand; Microbial Biotechnology Unit Faculty of Liberal Arts and Science Kasetsart University Nakhon Pathom Thailand; Functional Ingredients and Food Innovation Research Group (IFIG) National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA) Khlong Luang Thailand
Type
Article
Source Title
Mycology
ISSN
21501203
Year
2024
Open Access
All Open Access Gold
Publisher
Taylor and Francis Ltd.
DOI
10.1080/21501203.2024.2305719
Abstract
Spent mushroom substrate (SMS) a lignocellulosic waste after mushroom production is generally discarded without proper management. There is increasing interest in the sustainable transformation of lignocellulosic waste into high-value products. Within this context the present study investigated the potential of the SMS from the cultivation of Pleurotus pulmonarius and Auricularia auricula on rubber tree wood sawdust as substrates for xylooligosaccharides (XOS) production. SMS samples from these two edible mushrooms were extracted using alkaline xylan extraction producing maximum true recovery amounts of xylan in the range 34.61%�.49% using 18% NaOH at 70 ?C for 3 h. Production of XOS from alkaline-extracted xylan from the SMS samples of both mushroom species using economically crude xylanases from Aspergillus flavus KUB2 resulted in XOS (X2朮5) production of 241.47�9.04 mg/g with X3 as the predominant XOS product. The produced XOS had excellent prebiotic activity and 2 2?-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and contained high total phenolic contents. The combined beneficial bioactivities in terms of prebiotic and antioxidant properties suggested that the XOS produced from sawdust-based SMS samples of P. pulmonarius and A. auricula could be promising ingredients for both food and pharmaceutical applications. ? 2024 The Author(s). Published by Informa UK Limited trading as Taylor & Francis Group.
License
CC BY-NC
Rights
Authors
Publication Source
WOS