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Verrucosispora endophytica sp. Nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.)
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Document Title
Verrucosispora endophytica sp. Nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.)
Author
Ngaemthao W., Pujchakarn T., Chunhametha S., Suriyachadkun C.
Name from Authors Collection
Affiliations
BIOTEC Culture Collection, Biodiversity and Biotechnological Resource Research Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand
Type
Article
Source Title
International Journal of Systematic and Evolutionary Microbiology
ISSN
14665026
Year
2017
Volume
67
Issue
12
Page
5114-5119
Open Access
All Open Access, Bronze, Green
Publisher
Microbiology Society
DOI
10.1099/ijsem.0.002425
Format
Abstract
A novel endophytic filamentous bacterium strain A-T 7972T, was isolated from wild orchid Grosourdya appendiculata (Blume) Rchb.f. collected in Thailand. The bacterium developed single non-motile spores with warty surface on substrate mycelia. The taxonomic position was described using a polyphasic approach. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 7972T belonged to the genus Verrucosispora and shared the highest sequence similarity with V. lutea YIM 013T (98.71 %) and V. gifhornensis DSM 44337T (98.53 %). The values of DNA–DNA relatedness that distinguished this novel strain from its closest species were below 70 %. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars were mannose, ribose, glucose and xylose. The predominant menaquinone was MK-9(H4). The predominant fatty acids were branched fatty acids iso-C15: 0 and iso-C16: 0. The diagnostic phospholipids profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides. The G+C content of the genomic DNA was 70.5 mol%. Based on its phenotypic, chemotaxonomic and genotypic characteristics, the new isolate A-T 7972T (=BCC 50981T=TBRC 6031T=NBRC 112512T) is proposed to be the type strain of novel species, Verrucosispora endophytica sp. nov. © 2017 IUMS.
Industrial Classification
Knowledge Taxonomy Level 1
Knowledge Taxonomy Level 2
Knowledge Taxonomy Level 3
Funding Sponsor
International Centre for Genetic Engineering and Biotechnology; National Brain Research Centre
License
N/A
Rights
N/A
Publication Source
Scopus